VIC激发波长和发射波长

VIC激发波长和发射波长

VIC是一种荧光染料，初由Thermofisher 开发。VIC的分子量为1023.38，VIC激发波长(Ex)为526nm，发射波长(Em)为543nm。VIC发射在可见光谱的绿黄色部分。用于在5′端( 可作为探针在各种实时荧光 PCR、杂交和基于荧光的遗传分析中应用 )荧光标记寡核苷酸。VIC磷酰胺化物是将VIC标签掺入寡核苷酸中方便的构建块。

Figure 1：VIC激发波长和发射波长的光谱图

VIC is a fluorescent dye that was originally developed by Applied Biosystems (now, Thermofisher). VIC emits in the green-yellow part of the visible spectrum. It is used to fluorescently label oligonucleotides at the 5’-end (for use as probes in a variety of real-time PCR, hybridization, and fluorescence-based genetic analysis applications). VIC phosphoramidite is the most convenient building block to incorporate VIC tag into an oligonucleotide.

Molecular weight：1023.38

Excitation：526nm

Emission：543nm

Figure 2. Chemical structure for 5-VIC phosphoramidite.

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