紫外交联仪Stratalinker UV 2400
型号:ML29-Stratalinker UV 2400
紫外交联仪Stratalinker UV 2400特点:
为了使核苷酸固定在膜上,传统的方法是将膜置于真空烘箱中80度烘2小时,在紫外交联仪上仅需30-60秒
与真空烘焙相比,大大增加杂交信号强度
内部配备光度检测仪,可以补偿由于紫外灯泡老化造成的输出偏移
具自动交联、时间和能量三种模式供选择
带有显示时间、能量的显示器及设置面板
紫外交联仪Stratalinker UV 2400应用:
将核酸共价结合到固相支持物(如硝酸纤维素膜)上
Northem,Southern,dot及slot印迹分析
杂交芯片的制备
菌落、噬菌斑筛选,琼脂糖凝胶中DNA的切割
产生胸腺二聚体,用于部分限制性内切酶消化作图
紫外敏感性测试,用于鉴定recA宿主菌
样品紫外照射处理
诱导培养细胞的凋亡
照射波长:254nm(配8瓦254nm紫外线灯管5根)
内部尺寸:47.7W/34.3D/16.8H
紫外交联仪Stratalinker UV 2400的特点:
用于将 DNA 或 RNA 交联到涂层载玻片、尼龙、硝化纤维素或尼龙增强硝化纤维素膜上
将宝贵的时间从几个烤箱烘烤小时减少到30-60秒
与烤箱烘烤相比,增加杂交信号
内部光电探测器补偿老化UV灯泡的偏移输出
可在自动交叉、时间或能量模式下操作
安捷伦基因组学/Stratagene UV 交联剂设计用于将 DNA 或 RNA 交联到尼龙、硝酸纤维素或尼龙增强的硝化纤维素膜上。交联过程仅需25-50秒,而传统的方法是在80°C下烘烤过滤器2小时。此外,与烤箱烘烤相比,交联已被证明可以显着增加杂交信号。为了获得佳交联性能,每个Stratalinker紫外交联剂都配备了一个内部光电探测器,旨在补偿老化紫外线灯泡功率输出的自然变化。
Stratalinker UV紫外交联仪可用于Northern, Southern, dot or slot blot 分析,1-3菌落或斑块筛选,在印迹之前在琼脂糖凝胶中划伤DNA,4二聚体形成以进行部分消化以进行快速限制性映射,5用于宿主应变验证的UV灵敏度测试,6和PCR样品的紫外线照射。 时间模式或能量模式,该设备有两种方便的尺寸:用于较小膜的 Stratalinker 1800 UV紫外交联仪和用于较大膜的 Stratalinker 2400 UV紫外交联仪。
紫外交联仪Stratalinker UV 2400英文介绍:
Features:
For Crosslinking DNA or RNA to Coated Glass Slides, Nylon, Nitrocellulose or Nylon-Reinforced Nitrocellulose Membranes
Greatly Reduces Valuable Time From Several Oven Baking Hours to 30-60 Seconds
Increases Hybridization Signal Compared to Oven Baking
Internal Photodetector Compensates for Shifting Output of Aging UV Bulbs
Can be Operated in Autocrosslink, Time or Energy Modes
The Agilent Genomics / Stratagene UV crosslinker is designed to crosslink DNA or RNA to nylon, nitrocellulose, or nylon-reinforced nitrocellulose membranes. The crosslinking process takes only 25-50 seconds, in contrast to the traditional method of baking filters at 80°C for 2 hours. Additionally, crosslinking has been shown to significantly increase hybridization signals when compared to oven-baking. For optimal crosslinking performance, each Stratalinker UV crosslinker is equipped with an internal photodetector designed to compensate for the natural shift in power output of aging ultraviolet bulbs.
The Stratalinker UV crosslinker may be used for Northern, Southern, dot or slot blot analysis,1-3 colony or plaque screening, nicking of DNA in agarose gels prior to blotting, 4 dimer formation to perform partial digests for rapid restriction mapping,5 UV sensitivity testing for host strain verification,6 and UV irradiation of PCR samples.7 The Stratalinker UV crosslinker may be used in the Autocrosslink, Time, or Energy modes, and the apparatus is available in two convenient sizes-the Stratalinker 1800 UV crosslinker for smaller membranes and the Stratalinker 2400 UV crosslinker for larger membranes.