LUYOR-3415用于观察小鼠黑色素瘤转移

2022年10月重庆医科大学在《Biological Research》发表《Melanoma stem cells promote metastasis via exosomal miR-1268a inactivation of autophagy》文献，文献中实验采用了LUYOR-3415RG双波长荧光蛋白激发光源和LUV-50A荧光观察眼镜观察红色荧光蛋白RFP在小鼠肺部肿瘤细胞的上的表达。

To study the effect of miR-1268a overexpression on OL cells metastasis in vivo, we generated OL cells stably overexpressing miR-1268a via lentiviral infection (Fig. 4J). 5 次 10^{5} OE-NC-OL and OE-miR-1268a-OL cells were injected into NOD/SCID mice via the tail vein, respectively (Sect. “Materials and methods”). All mice were sacrificed at day 35 after tumor cell injection. Since OE-NC-OL and OE-miR-1268a-OL cells were RFP-labeled, macroscopic metastatic foci formed at the lung could be visualize under external fluorescence imaging using LUYOR-3415 Dual Fluorescent Protein Excitation Light Source and LUV-50A Glasses. As shown in Fig. 4K, injected OE-miR-1268a-OL cells produced significantly more macroscopic lung foci than the OE-NC-OL cells (n = 6). Subsequently, H&E analysis was performed on the largest cross-sectional lung sections of the two groups of mice. Statistical analysis showed that there were significantly more microscopic metastatic foci in the lungs of mice treated with OE-miR-1268a-OL cells (The black arrows show the formed metastases) (Fig. 4L). These observations show that miR-1268a can enhance metastatic colonization efficiency of OL cells that are oligometastatic. So far, we have demonstrated that miR-1268a, transferred from OL-SCs-derived exosomes to OL cells, can effectively augment the clonogenic colonization capability of OL cells to give arise to more extensive macroscopic metastases.

文献地址：https://biolres.biomedcentral.com/articles/10.1186/s40659-022-00397-z 

上海峰志仪器有限公司现货供应美国路阳生产的LUYOR-3415RG手持式双波长荧光蛋白激发光源，能够观察绿色荧光蛋白和1红色荧光蛋白在小鼠、斑马鱼、线虫等模式动物上的表达。

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1.便携式荧光蛋白激发光源LUYOR-3415RG

2.LUYOR-3415rg激发光源应用案例

重庆医科大学介绍：

重庆医科大学创建于1956年，由上海医学院（现复旦大学上海医学院）分迁来渝组建，原名重庆医学院，1985年更名为重庆医科大学。学校是国务院学位委员会批准的首批具有博士和硕士学位授予权的单位，是教育部批准的首批来华留学示范基地。学校2015年成为重庆市人民政府、卫生健康委员会（原卫生计生委）和教育部共建高校，2016年成为“高等学校学科创新引智计划”（“111计划”）首批立项的地方高校之一。学校由位于渝中区的袁家岗校区和位于重庆大学城的缙云校区组成，校园总面积2650亩，其中缙云校区占地2100亩。校园环境优美，风景如画。

重庆医科大学现有全日制在校学生26810人，其中博士、硕士研究生7124人，本科生18824人，专科生258人（对口支援少数民族项目），留学生604人。学校具有招收博士后研究人员，港澳台学生和外国留学生资格。学校现有8个一级学科博士学位授权点、13个一级学科硕士学位授权点，3个博士专业学位授权点、11个硕士专业学位授权点；4个重点学科，8个学科进入ESI全球前1%（2022年1月科睿唯安数据），4个重庆市学科，16个重庆市“十四五”市级重点学科。

文章转自：https://www.luyoruv.com/news/news1/673.html