荧光适配器用于斑马鱼代谢疾病研究

荧光适配器用于斑马鱼代谢疾病研究

体视显微镜荧光适配器用于mCherry和eGFP在斑马鱼上的表达

马里兰大学巴尔的摩分校使用美国NIGHTSEA的SFA体视显微镜荧光适配器系统用于筛选他们的转基因斑马鱼。该研究小组研究了与代谢性疾病相关的基因的功能，包括糖尿病，肥胖症和NAFLD / NASH（非酒精性脂肪性肝病/非酒精性脂肪性肝炎）。产生胰岛素的β（β）细胞发生在胰腺的胰岛中，在维持葡萄糖水平方面至关重要。它们是代谢疾病社区的一个重要研究课题，斑马鱼β细胞在功能和发育方面都与哺乳动物相似。在我们观察的幼虫发育阶段，Dunleavey博士估计胰岛含有大约35 β细胞。斑马鱼模型表达了两种荧光报告基因—— 胰腺外分泌的eGFP和β细胞中的mCherry（ptf1a：eGFP和ins：mCherry，也称为exocrine eGFP和β细胞mCherry）——Dunleavey博士希望能够同时看到它们。

下面的图像是用iPhone手持在配备美国nightsea SFA的尼康SMZ1000体视显微镜的目镜上快速制作的。SFA-RB荧光适配器皇家蓝激发/发射组用于成像eGFP，SFA-GR荧光适配器绿色组用于成像mCherry。上海峰志仪器有限公司代理销售美国nightsea公司生产的荧光适配器。

斑马鱼在白光照射下的图像

斑马鱼在SFA-RB皇家蓝激发下eGFP发出绿色荧光

斑马鱼在SFA-GR荧光适配器绿色激发下mCherry发出的红色荧光

以下为引用的英文原文：

Zebrafish Metabolic Disease Reporters – mCherry and eGFP

NIGHTSEA recently visited Dr. Jessica Dunleavey at the Zaghloul Lab at the University of Maryland Baltimore to determine whether our Model SFA Stereo Microscope Fluorescence Adapter system could be used to screen their transgenic zebrafish. The research group investigates the functions of genes associated with metabolic diseases including diabetes, obesity, and NAFLD/NASH (nonalcoholic fatty liver disease / nonalcoholic steatohepatitis). Insulin-producing β (beta) cells occur in islets in the pancreas  and are critically important in maintaining glucose levels. They are a significant study topic in the metabolic disease community, and zebrafish β cells are similar to those in mammals both functionally and developmentally. At the stage of larval development we looked at, Dr. Dunleavey estimated that the islet contained approximately 35 β cells. The zebrafish model expresses two fluorescent reporters – eGFP in the exocrine pancreas and mCherry in the β cells (ptf1a:eGFP and ins:mCherry, also known as exocrine eGFP and β cell mCherry) – and Dr. Dunleavey hoped to be able to see both of them.

The test was successful. The images below were made quickly with an iPhone hand-held over the eyepiece of a Nikon SMZ1000 stereo microscope outfitted with the NIGHTSEA SFA. The Royal Blue excitation/emission set was used for imaging eGFP, and the Green set for imaging mCherry.

上海峰志仪器有限公司代理销售美国nightsea、美国路阳生产的体视显微镜荧光适配器和荧光手电筒。有关体视显微镜荧光适配器产品介绍请浏览《美国nightsea荧光适配器 》，上海峰志仪器有限公司销售的荧光观察设备能够观察植物愈伤、叶片、种子、根系等上面的转基因的表达，还能观察转基因在动物如老鼠、斑马鱼、果蝇等实验动物上的表达。有便携式单波长荧光手电筒，也有手持式大面积照射高强度的双波长激发光源，上海峰志提供免费试机，有兴趣的老师和同学可按照网页底部联系联系。