蓝色荧光蛋白TagBFP

蓝色荧光蛋白TagBFP

蓝色荧光蛋白TagBFP又名 mTagBFP，蓝色荧光蛋白TagBFP- 亮蓝色荧光 - 在融合中成功表现的单体蛋白 - 成熟快，光稳定性高 - 极高的pH稳定性，蓝色荧光蛋白的激发光波长为402nm，发射光波长为457nm。

TagBFP是2008年发表的一种基本（组成型荧光）蓝色荧光蛋白，来源于Entacmaea quadricolor。据报道，它是一种非常快速成熟的单体，具有非常低的酸敏感性。

TagBFP a.k.a. mTagBFP，TagBFP is a basic (constitutively fluorescent) blue fluorescent protein published in 2008, derived from Entacmaea quadricolor. It is reported to be a very rapidly-maturing monomer with very low acid sensitivity.

蓝色荧光蛋白TagBFP的光谱图

蓝色荧光蛋白用什么光激发？

从以上蓝色荧光蛋白的激发和发射光谱图可以看出，蓝色荧光蛋白用紫光激发，发出蓝色荧光。如果您用便携式荧光蛋白激发光源，建议使用LUYOR-3280VI以及LUYOR-3415的V波段。

Blue fluorescent protein TagBFP

- Bright blue fluorescence

- Monomeric protein with successful performance in fusions

- Fast maturation, high photostability

- Extremely high pH-stability

- Recommended for protein labeling, acidic organelle labeling, FRET applications

TagBFP (scientific name mTagBFP) is a monomeric blue fluorescent protein generated by site-specific and random mutagenesis of TagRFP [Subach et al., 2008]. TagBFP possesses bright blue fluorescence with excitation/emission maxima at 402 and 457 nm, characterized by high photostability and extremely high pH-stability.

Compared to EBFP2 [Ai et al., 2007], TagBFP is more then 1.8 times brighter, much more pH-stable and has twice shorter maturation half-time at 37°C. Narrow fluorescence emission peak of TagBFP provides for accurate and easy spectral separation with cyan and green fluorescent proteins and makes it a preferable tag for multicolor labeling.

Good overlap between the emission spectrum of TagBFP and the absorbance spectra of TagGFP2 allows using these two proteins as a FRET pair.

如果要观察蓝色荧光蛋白在斑马鱼上的表达，可以选用LUYOR-3421VI 台式激发光源配合体视显微镜观察。LUYOR-3421VI 台式激发光源安装有405-415nm的激发模组，能够激发BFP荧光蛋白发出蓝色荧光。