橙色荧光蛋白mOrange激发光谱
mOrange是2004年发表的一种碱性(组成型荧光)橙色荧光蛋白,来源于Discosoma sp.。它具有高酸敏感性。mOrange is a basic (constitutively fluorescent) orange fluorescent protein published in 2004, derived from Discosoma sp.. It has high acid sensitivity.
morange橙色荧光蛋白的激发光的中心波长为548nm,发射光的中心波长为562nm。
橙色荧光蛋白mOrange激发光谱图:
Orange fluorescent protein Proteins
Orange fluorescent protein (OFP) is a versatile biological marker used to monitor physiological processes, visualize protein localization, and detecting transgenic expression in vivo. OFPSparkTM is a red (orange) fluorescent protein (excitation/emission maxima are 549 and 566 nm, respectively) derived from DsRed. Possessing high photostability and pH stability, OFPSparkTM is more than twice brighter than mOrange2. OFPSparkTM can be expressed and detected in a wide range of organisms.
橙色荧光蛋白 (OFP) 是一种多功能生物标记物,用于监测生理过程、可视化蛋白质定位和检测体内转基因表达。OFPSparkTM 是一种源自 DsRed 的红色(橙色)荧光蛋白(激发/发射zui大值分别为 549 和 566 nm)。OFPSparkTM 具有高光稳定性和 pH 稳定性,比 mOrange2 亮两倍以上。OFPSparkTM 可以在多种生物体中表达和检测。
什么是mOrange2荧光蛋白?
mOrange2 fluorescent protein. Living Colors mOrange2 is an extremely bright orange fluorescent protein monomer ideal for use as a tag or reporter. The mOrange2 fluorescent protein mutant derived from a monomeric mutant of DsRed, which was developed in Dr. Roger Tsien’s lab (Nathan C Shaner et al. 2004; Wang et al.
常用荧光蛋白的激发和发射光:
荧光蛋白 | 蛋白型号 | 激发波长(EX) | 发射波长(EM) |
青色荧光蛋白 | AmCyan1 | 458nm | 489nm |
绿色荧光蛋白 Green fluorescent proteins | AcGFP1 | 475nm | 505 nm |
ZsGreen1 | 493nm | 505nm | |
黄色荧光蛋白 Yellow fluorescent proteins | ZsYellow1 | 529nm | 539 nm |
mBanana | 540nm | 553 nm | |
桔色荧光蛋白 Orange fluorescent proteins | mOrange2 | 549nm | 565nm |
红色荧光蛋白 | DsRed-Express2 | 554nm | 591nm |
DsRed-Express | 554nm | 586nm | |
tdTomato | 554nm | 581 nm | |
DsRed-Monomer | 557nm | 592 nm | |
DsRed2 | 563nm | 582 nm | |
AsRed2 | 576nm | 592 nm | |
mStrawberry | 574nm | 596 nm | |
mCherry | 587nm | 610nm |
EX: Excitation max (nm)
EM: Emission max (nm)
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